Deleted
Sample Discontinued
Experience: None
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Part:BBa_M36118

Designed by: Samson Phan, Noriko Ishisoko   Group: Stanford BIOE44 - S11   (2012-05-29)


GULO enzyme with start and stop

This construct includes the kozacs sequence, the sequence for the L-gulonolactone oxidase enzyme, and a stop codon. This is a standalone part, ie, if this is the only enzyme you want to produce, then this sequences will allow you to do this with minimal work.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1307
    Illegal PstI site found at 558
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1307
    Illegal PstI site found at 558
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1307
    Illegal BamHI site found at 283
    Illegal BamHI site found at 1286
    Illegal XhoI site found at 993
    Illegal XhoI site found at 1317
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1307
    Illegal PstI site found at 558
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1307
    Illegal PstI site found at 558
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
None